Saturday, March 21, 2020

Free Essays on The Fall Of Fidel Castro

Fidel collapsed today before thousands at an outdoor rally. This is a reminder to all sides of the political struggle that no one is immortal. Fidel Castro is an old man who is closer to death than he was forty years ago. His rambling speech broadcast over Telemundo recently, and passing out at a public forum serves as evidence to back up the previous sentence. This brings us to one of the lies spread by the regime that has been imbibed by almost everyone on the planet, and especially Cuban exiles. The myth is that nothing will change in Cuba until Fidel Castro dies, then the system will end with him. If I believed that to be true I would’ve never gotten involved in the struggle for a free Cuba, and sat around waiting for biology to take its course. However, it is not true. If we look back through the history of tyrants: Lenin, Stalin, Mao, Papa Doc, the Somozas, Kim il Sung, Ho Chi Minh, and a list tragically too long to list here, then you’ll find that when tyrants di e these evil tyrannies have replacements that are equally as evil. Let me provide two notorious examples. The first tyrant of the Soviet Union was Lenin. During Lenin’s tenure outsiders could even claim that the regime had moderated with its New Economic Policy that allowed foreign investment and limited amounts of capitalism to keep the regime from collapsing completely. Did this early engagement of trade with the Soviet Union in the 1920s have a big pay off? Was there a turn to democracy or greater economic liberalization? Lenin died a transition or should I say succession took place. The answer to the previous questions is given with the name Josef Stalin and the murder of 50 million Russians at the hand of their own government. Communism didn’t fall in the Soviet Union or Eastern Europe because of the death of a tyrant, or because of economic engagement. Communism didn’t fall it was pushed. It was knocked down abroad by the effective foreign policy o... Free Essays on The Fall Of Fidel Castro Free Essays on The Fall Of Fidel Castro Fidel collapsed today before thousands at an outdoor rally. This is a reminder to all sides of the political struggle that no one is immortal. Fidel Castro is an old man who is closer to death than he was forty years ago. His rambling speech broadcast over Telemundo recently, and passing out at a public forum serves as evidence to back up the previous sentence. This brings us to one of the lies spread by the regime that has been imbibed by almost everyone on the planet, and especially Cuban exiles. The myth is that nothing will change in Cuba until Fidel Castro dies, then the system will end with him. If I believed that to be true I would’ve never gotten involved in the struggle for a free Cuba, and sat around waiting for biology to take its course. However, it is not true. If we look back through the history of tyrants: Lenin, Stalin, Mao, Papa Doc, the Somozas, Kim il Sung, Ho Chi Minh, and a list tragically too long to list here, then you’ll find that when tyrants di e these evil tyrannies have replacements that are equally as evil. Let me provide two notorious examples. The first tyrant of the Soviet Union was Lenin. During Lenin’s tenure outsiders could even claim that the regime had moderated with its New Economic Policy that allowed foreign investment and limited amounts of capitalism to keep the regime from collapsing completely. Did this early engagement of trade with the Soviet Union in the 1920s have a big pay off? Was there a turn to democracy or greater economic liberalization? Lenin died a transition or should I say succession took place. The answer to the previous questions is given with the name Josef Stalin and the murder of 50 million Russians at the hand of their own government. Communism didn’t fall in the Soviet Union or Eastern Europe because of the death of a tyrant, or because of economic engagement. Communism didn’t fall it was pushed. It was knocked down abroad by the effective foreign policy o...

Thursday, March 5, 2020

How To Streak a Bacterial Culture

How To Streak a Bacterial Culture Bacterial culture streaking allows bacteria to reproduce on a culture medium in a controlled environment. The process involves spreading bacteria across an agar plate and allowing them to incubate at a certain temperature for a period of time. Bacterial streaking can be used to identify and isolate pure bacterial colonies from a mixed population. Microbiologists use bacterial and other microbial culture streaking methods to identify microorganisms and to diagnose infection. What You Need: Culture plate with microorganismsInoculating loop or sterile toothpicksAgar platesBunsen burner or another flame producing instrumentGlovesTape Heres How: While wearing gloves, sterilize an inoculating loop by placing it at an angle over a flame. The loop should turn orange before you remove it from the flame. A sterile toothpick may be substituted for the inoculating loop. Do not place toothpicks over a flame.Remove the lid from a culture plate containing the desired microorganism.Cool the inoculating loop by stabbing it into the agar in a spot that does not contain a bacterial colony.Pick a colony and scrape off a little of the bacteria using the loop. Be sure to close the lid.Using a new agar plate, lift the lid just enough to insert the loop.Streak the loop containing the bacteria at the top end of the agar plate moving in a zig-zag horizontal pattern until 1/3 of the plate is covered.Sterilize the loop again in the flame and cool it at the edge of the agar away from the bacteria in the plate that you just streaked.Rotate the plate about 60 degrees and spread the bacteria from the end of the first streak into a second area using th e same motion in step 6. Sterilize the loop again using the procedure in step 7.Rotate the plate about 60 degrees and spread the bacteria from the end of the second streak into a new area in the same pattern.Sterilize the loop again.Replace the lid and secure with tape. Invert the plate and incubate overnight at 37 degrees Celsius (98.6 degrees Fahrenheit).You should see bacterial cells growing along the streaks and in isolated areas. Tips: When sterilizing the inoculating loop, make sure that the entire loop turns orange before using on the agar plates.When streaking the agar with the loop, be sure to keep the loop horizontal and only streak the surface of the agar.If using sterile toothpicks, use a new toothpick when performing each new streak. Throw all used toothpicks away. Safety: When growing bacterial colonies, you will be dealing with millions of bacteria. It is important that you follow all lab safety rules. Precautions should be taken to ensure that you dont inhale, ingest, or allow these germs to touch your skin. Bacterial plates should be kept closed and secured with tape while incubating. Any unwanted bacterial plates should be disposed of properly by placing them in an autoclave to kill the bacteria before discarding them. Household bleach may also be poured over the bacterial colonies to destroy them.